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KMID : 1005220070220030155
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Journal of Embryo Transfer
2007 Volume.22 No. 3 p.155 ~ p.160
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Effects of Culture Media and Oxygen Concentration on In Vitro Development of Porcine IVM/IVE Embryos
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Choe Chang-Yong
Cho Sang-Rae
Choi Sun-Ho
Kim Hyun-Jong
Han Man-Hye
Kang Da-Won
Shin Yong-Won
Han Jae-Hee
Son Dong-Soo
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Abstract
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During in vitro culture of mammalian oocytes and embryos, the cells are exposed to the risks that cause cell injury or death. Numerous studies have been reported that the cell injury may be induced by the action of free radicals generated by auto-oxidation. This study was undertaken to investigate the optimal culture condition system for in vitro culture of porcine embryos. We first evaluated the effect of culture media on the porcine embryo development. NCSU-23 and PZM-5, culture medium tested, were failed to produce significant difference on the rate of blastocyst formation. In NCSU-23, the developmental rate was slightly higher than that in PZM-5. During in vitro maturation (IVM), fertilizaton (IVF), and culture (IVC) under 5 or 20% oxygen (O_2), the rates of cleavage and development were insignificantly different from each other under our culture condition (20% O_2, in NCSU-23), the mean cell number per blastocyst was 40{pm}10. These results showed that medium and $O_2$ concentration had no significant effect on the development of porcine embryos.
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KEYWORD
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culture media, oxygen concentration, porcine, blastocyst
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